Investigation of pancreatic digestive enzymes changes from egg to larvae phase of Schizothorax zarudnyi

In order to identify trends and provide suitable diet for Schizothorax zarudnyi, the activity of alpha-amylase, trypsin, and lipase in five treatment groups including: fertilized egg, newly-hatched larvae, the larvae a day, larvae absorbed in the bag 2/ 3 yolk and absorption of yolk sac larvae, in 20 embryonated eggs and 80 larvae at the end of 53 days were studied. Analysis of the data showed that in all five groups of fish eggs and larvae, the activity of the alpha-amylase enzyme is even more. Most of the activity of the enzyme alpha-amylase in the bottom of the absorption of the yolk sac (59. 63±4. 4 IU/ mg protein (and lowest in the 2/3 absorb the yolk sac (7. 78±0. 5 IU/ mg protein) seen. The activity of alpha-amylase enzyme in the process of absorbing the yolk sac in comparison with the other groups were significantly different (p < 0. 05). Results showed that the activity of the trypsin and lipase enzyme from egg to larvae four-day rising trend, and highest of their activities observed at the end of yolk sac absorption stage. In assessing enzymes trypsin and lipase were not observed significant difference between the 3-day-old larvae and 4-day-old larvae (p > 0/05), but the larvae 4 days fertilized eggs and newly hatched larvae were significant differences (p < 0/05).